human epidermal keratinocyte cells heks  (Procell Inc)

Journal: bioRxiv

Article Title: StrataChip: a microphysiological system capturing dynamic keratinocyte fate and mechanical transitions during human epidermal morphogenesis

doi: 10.64898/2026.03.26.714483

Figure Lengend Snippet: (a) 3D schematic and section rendering of the StrataChip highlighting the central port housing the epidermal tissue and dermal equivalent hydrogel surrounded by media channels. PDMS, polydimethylsiloxane. (b) Photograph of the StrataChip. Scale bar 5 mm. (c) Stepwise fabrication of StrataChip using photolithography and soft lithography to generate a PDMS device that is bonded to a coverslip (d) StrataChip central port treatment with polydopamine (PDA) and measurements of device features. ( e) Time course of fibroblast-collagen hydrogel contraction, indicated by dashed lines, in the central port of PDA treated devices versus vehicle treated devices. Scale bar 0.5 mm. (f) Quantification of fibroblast collagen hydrogel contraction, by percent coverage of central port, in PDA treated devices versus vehicle treated devices. P = 0.01073 (Day 5); 0.00095 (Day 6); 0.00030 (Day 7); 0.00005 (Day 8) with Holm-Sidak multiple Student’s t -test; N = 4 independent devices for each group. (g) Development timeline of StrataChip: seeding of dermal equivalent (1, 2) followed by seeding of human epidermal keratinocytes (3, 4) and introduction of air-liquid interface (ALI) (5). Confocal immunofluorescence image of epidermal tissue and dermal hydrogel with fibroblasts (indicated by arrows). Visualized with phalloidin f-actin stain in orange. (6). Scale bar, 20 µm.

Article Snippet: Human epidermal keratinocytes (Ker-CTs) cells were obtained from ATCC.

Techniques: Immunofluorescence, Staining

Journal: bioRxiv

Article Title: StrataChip: a microphysiological system capturing dynamic keratinocyte fate and mechanical transitions during human epidermal morphogenesis

doi: 10.64898/2026.03.26.714483

Figure Lengend Snippet: (a) Fluorescence micrographs showing the epidermis developmental timeline in orthogonal slices at day 2, day 5 and day 7. Visualized with phalloidin f-actin stain in orange. (b) Bar graph of the average epidermal thickness. N = 15 independent devices. (c) Representative illustration of epidermis with colors representing expression of differentiation markers in basal cells (BC), spinous cells (SC) and granular cells (GC) within epidermal layers: Magenta-Keratin 14 (K14), Green-Keratin 10 (K10), Orange-Involucrin (IVL). (d) Orthogonal slice fluorescence micrograph of StrataChip epidermis at day 7 immunostained with differentiation markers: KRT14 (left), KRT10 (left and right), and IVL (right), as well as DNA stain DAPI. (e) Quantification of the layer-specific expression for epidermal differentiation markers KRT14, KRT10, and IVL. N ≥ 4 for each marker. (f) Orthogonal slice fluorescence micrograph of epidermis immunostained for p63. Dashed line represents cell-ECM boundary. (g) Flowchart of epidermal tissue dissociation, single-cell RNA-sequencing, and analysis. ( h) Uniform manifold approximation and projection (UMAP) plot of cell types identified in StrataChip tissues. (i) Expression UMAP with representative gene expression for each layer-specific cell type. Dark to light pink indicates high to low gene expression. (j) Dot plot showing representative gene expression for each cell type in StrataChip tissues. (k) Three representative gene ontology (GO) terms for each keratinocyte category. All scale bars, 20 µm.

Article Snippet: Human epidermal keratinocytes (Ker-CTs) cells were obtained from ATCC.

Techniques: Fluorescence, Staining, Expressing, Marker, Single Cell, RNA Sequencing, Gene Expression

Journal: bioRxiv

Article Title: StrataChip: a microphysiological system capturing dynamic keratinocyte fate and mechanical transitions during human epidermal morphogenesis

doi: 10.64898/2026.03.26.714483

Figure Lengend Snippet: (a)-(d) Top ten relevant Gene Ontology (GO) terms from the cell fates: fibroblasts and basal, spinous, and granular keratinocytes.

Article Snippet: Human epidermal keratinocytes (Ker-CTs) cells were obtained from ATCC.

Techniques:

Journal: bioRxiv

Article Title: StrataChip: a microphysiological system capturing dynamic keratinocyte fate and mechanical transitions during human epidermal morphogenesis

doi: 10.64898/2026.03.26.714483

Figure Lengend Snippet: ( a) Differentially expressed genes between basal I versus basal II keratinocytes. (b) Differentially expressed genes between spinous I versus spinous II keratinocytes. (c) and (d) Enriched GO terms of spinous I and spinous II keratinocytes when compared with each other. (e) Pseudotime analysis of cell clusters from StrataChip.

Article Snippet: Human epidermal keratinocytes (Ker-CTs) cells were obtained from ATCC.

Techniques:

Journal: Pharmaceutics

Article Title: Valorization of Thyme Combined with Phytocannabinoids as Anti-Inflammatory Agents for Skin Diseases

doi: 10.3390/pharmaceutics17101291

Figure Lengend Snippet: Gene expression heat maps in PMA-induced HaCaT cells. Heat maps illustrating the expression levels of pro-inflammatory ( A ), anti-inflammatory ( B ), and cannabinoid receptor ( C ) genetic markers in HaCaT keratinocyte cells subjected to PMA-induced stress, following treatment with FA, FB, FC, FA.Cann, FB.Can, and FC.Cann. Gene expression changes are shown as average Log 2 (fold change) values (n = 2) relative to the non-stimulated control ( C ), with the following: C: Control group (unstimulated; untreated); S+NT: stressed (PMA-induced); non-treated group; S+T: stressed and treated group. Color scale: Red indicates gene upregulation, while Blue reflects downregulation. Normalization was performed using 18S rRNA as the internal housekeeping gene.

Article Snippet: Human epidermal keratinocytes (HaCaTs) (CLS 300493) were grown in Dulbecco’s Modified Eagle Medium (DMEM) with high glucose (4500 mg/L), supplemented with 10% ( v / v ) fetal bovine serum (FBS), 100 IU/mL of penicillin and 100 μg/mL of streptomycin, and later designated as complete culture medium.

Techniques: Gene Expression, Expressing, Control

Journal: PLOS One

Article Title: 3-O-acetylrubiarbonol B preferentially targets EGFR and MET over rubiarbonol B to inhibit NSCLC cell growth

doi: 10.1371/journal.pone.0329706

Figure Lengend Snippet: (A) Viability of NSCLC cells (HCC827 and HCC827GR) treated for 24 (black) and 48 h (white) with Ru-B (2, 4, 6, and 8 µM), ARu-B (2, 4, and 6 µM), gefitinib (GEF, 1 µM), and savolitinib (SAV, 2 nM) as monitored by the MTT assay. Data are shown as the mean ± SD (n = 3). (B) Cell viability of HEKa cells treated as in (A). (C) and (D) The soft agar assay was used to determine anchorage-independent colony growth in NSCLC cells (14 days of incubation). (C) Micrograph of the cells on day 14 and (D) colony number. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to vehicle only. Scale bar, 400 µm.

Article Snippet: HCC827 (GEF-sensitive NSCLC cell line) and HEKa (Human epidermal keratinocytes) cells were purchased from the ATCC (American Type Culture Collection, Manassas, USA).

Techniques: MTT Assay, Soft Agar Assay, Incubation